DNA Polymerases
Enzo Life Sciences AMPIGENE Taq DNA Polymerase (500U)
AMPIGENE Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to optimize PCR. The AMPIGENE Taq DNA Polymerase and 5x AMPIGENE reaction buffer are separate for flexibility and end-user customization. It can be used for routine PCR applications including genotyping, screening, and library construction. Applications: PCR.
LAMDA BIOTECH INC Conquest™ PCR Master Mix - 3
Thermus aquaticus DNA Polymerase (Taq DNA Polymerase) is a thermostable enzyme which can replicate DNA at 74oC and remain active even after incubation at 95oC. It has very strong 5’-3’ DNA polymerase activity with a fast extension rate. Although it lacks any 3’-5’ exonuclease activity (and therefore proofreading ability), Taq DNA polymerase remains one of the most widely used enzymes in molecular biology. Lamda Biotech’s bacterially derived Taq DNA Polymerase is unique for strong activity and low background in DNA amplification. This enzyme is purified from bacteria. Users should be cautious when dealing with bacteria DNA sequences.
New England Biolabs, Inc. Q5U™ Hot Start High-Fidelity DNA Polymerase – 100 units
Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.
Braintree Scientific Accuris Hot Start Taq, DNA Polymerase, 500u (5u/ul)
Hot Start Taq DNA Polymerase and Master Mix Exceptional sensitivity for low copy PCR Ideal for multiplex PCR and amplification of GC-rich DNA Same enhanced features as Accuris Taq Polymerase Buffer optimized for fast cycling and reproducibility Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification. When samples are ready, the reaction mixture is heated to 95C to denature the antibody and initiate the reaction. Similar to the standard Accuris Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without a red gel loading dye. Researchers preferring a chemically modified Hot Start polymerase, should order Accuris Hot Start ChemMod. **Sold for research purposes only and not for human use**
Braintree Scientific Accuris High Fidelity DNA Polymerase, 200 units
Accuris™ High Fidelity DNA Polymerase 50X higher fidelity than Taq DNA polymerase. Works with crude DNA samples. Ideal for cloning, mutagenesis and microarrays. Optimized buffer system with unique PCR enhancers. For applications requiring highly accurate amplification, choose Accuris High Fidelity DNA Polymerase. Modified for better sensitivity and higher activity for more reproducible performance, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples. A 3-5 proofreading exonuclease activity and an error rate of 4.55x10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets. **Sold for research purposes only and not for human use**
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase from Thermus aquaticus with 10x PCR reaction buffer containing MgCl2 | 9012-90-2 | MFCD00166026 | 250UN
Taq DNA Polymerase from Thermus aquaticus with 10x PCR reaction buffer containing MgCl2 | 9012-90-2 | MFCD00166026 | 250UN
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase from Thermus aquaticus with 10x PCR reaction buffer containing MgCl2 | 9012-90-2 | MFCD00166026 | 5KU
Taq DNA Polymerase from Thermus aquaticus with 10x PCR reaction buffer containing MgCl2 | 9012-90-2 | MFCD00166026 | 5KU
Alkali Scientific Radiant™ Hot Start Taq DNA Polymerase for FAM & SYBR green dye users constructing qPCR mix, 250 Reactions, (1 x .05mL Hot-Start Taq Polymerase, 1 x 1.5mL 20X KCI Reaction Buffer)
Radiant™ Hot Start Taq DNA Polymerase for FAM & SYBR green dye users constructing qPCR mix Radiant™ Hot-Start Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. Radiant™ Hot-Start Taq DNA Polymerase is especially suited to application where absence of residual host bacterial DNA is critical. Our HS Taq has an error rate of approximately 1 error per 2.0 x 105 nucleotides incorporated. PCR products generated with Radiant™ Hot-Start Taq Polymerase are A-tailed and may be cloned into TA cloning vectors.
New England Biolabs, Inc. Hot Start Taq DNA Polymerase – 1000 units
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
New England Biolabs, Inc. Hot Start Taq DNA Polymerase – 200 units
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
New England Biolabs, Inc. Phusion Hot Start Flex DNA Polymerase – 500 units
Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase, Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5' to 3' polymerase activity, 3' to 5' exonuclease activity and will generate blunt-ended products. The addition of an aptamer-based inhibitor allows room temperature reaction setup. Phusion Hot Start Flex DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates.
New England Biolabs, Inc. Q5U™ Hot Start High-Fidelity DNA Polymerase – 500 units
Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5 High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.